Deuterium raman imaging for lipid analysis

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August undefined, 2024

WebDec 27, 2024 · Quantitative methods to precisely measure cellular states in vivo have become increasingly important and desirable in modern biology. Recently, stimulated Raman scattering (SRS) microscopy has emerged as a powerful tool to visualize small biological molecules tagged with alkyne (C≡C) or carbon–deuterium (C–D) bonds in the … WebDeuterium Raman imaging for lipid analysis. @article{Egoshi2024DeuteriumRI, title={Deuterium Raman imaging for lipid analysis.}, author={Syusuke Egoshi and …

Deuterium-labeled Raman tracking of glucose accumulation and

WebJun 22, 2024 · a, Lipid intensity map at 2,845 cm −1 and selected ROIs in a wild-type adult worm. The images in c and e–g correspond to the red box in a.b, Retrieved Raman spectra of the selected regions of ... WebMar 30, 2015 · We also performed Raman imaging of lipid membrane ... Osaka University) for his support for the analysis of Raman imaging data. We thank Dr. Taisuke Ota (Nanophoton) for technical support for the … hide sheets bar in excel

Deuteration of terminal alkynes realizes simultaneous live cell …

WebAbstract. We generated a highly deuterated cholesterol analog (D38-cholesterol) and demonstrated its use for selective vibrational imaging of cholesterol storage in mammalian cells. D38-cholesterol produces detectable signals in stimulated Raman scattering (SRS) imaging, is rapidly taken up by cells, and is efficiently metabolized by acyl-CoA ... WebOct 1, 2024 · Deuterium Raman imaging for lipid analysis Egoshi et al. 3 www.sciencedirect.com Current Opinion in Chemical Biology 2024, 70: 102181 number … WebRaman-based optical imaging is a promising analytical tool for noninvasive, label-free chemical imaging of lipid bilayers and cellular membranes. Imaging using spontaneous … how far am i from lithonia ga

Deuterated Fatty Acid - an overview ScienceDirect Topics

WebFeb 1, 2024 · Fu et al. [7] reported that two classes of neutral lipid molecules, triacylglycerols (TAG) and cholesteryl esters (CE) labeled with deuterium at the single-lipid droplet level, could be distinguished in yeast, C. elegans, mammalian cells, and mouse tissues by hyperspectral stimulated Raman scattering (hsSRS) imaging method. … WebOct 2, 2012 · Figure 1. Representative Raman spectra of pure (A) d31-palmitic acid, (B) d33-oleic acid, and (C) d6-2,2,3,4,4,6-cholesterol. Most pronounced scattering intensities originate from CD2 and CD3 stretching vibrations between 2000 and 2300 cm−1. Specific CC bands are observed at 1622 cm−1 (for the deuterated fatty acids) and at 1653 cm−1 … hide sheets tableauWebApr 10, 2024 · The Raman shift was first set to 2,870 cm −1, to target the CH 2 stretching vibrational mode, imaging the skin's microscale lipid structure. Next, the Raman shift was automatically toggled to 2,250 cm −1 to target the nitrile stretch of Rux. Imaging was performed using a 20 × 0.8 NA microscope objective (UPLXAPO20X, Olympus, Tokyo, … hide shirt idleon

"WebJul 2, 2024 · Raman microscopy has been used to deduce information about the distributions of endogenous biomolecules without exogenous labeling. Several functional … " - Deuterium raman imaging for lipid analysis

Deuterium raman imaging for lipid analysis

WebJan 14, 2024 · Temporal changes in averaged Raman spectra. The A. nidulans hyphal cell we studied in this work is shown in Fig. 1a. The cell culture and Raman imaging … WebOct 1, 2024 · Therefore, deuterium has been used as a Raman tag for in vivo Raman imaging of lipids composed of many C–H bonds. In particular, among the deuterium …

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WebJul 28, 2024 · Raman microscopy is a powerful imaging tool for observing endogenous biomolecules, as it can detect molecular vibrations in cells without exogenous labeling. 1 … WebMay 4, 2024 · Alkyne-tagged Raman probes have shown high promise for noninvasive and sensitive visualization of small biomolecules to understand their functional roles in live …

WebFigure 1. Deuterium Raman imaging. [1] Our Services Lipid Analysis. Since deuterium is a stable isotope of hydrogen, deuterium labeling has little effect on the biological activity of the target. Therefore, BOC Sciences utilizes deuterium-labeled fatty acids, sterols, choline and phospholipids in combination with Raman imaging for lipid analysis. WebAug 16, 2024 · Here, a phenotypic platform that rapidly produces AST results by femtosecond stimulated Raman scattering imaging of deuterium oxide (D 2 O) metabolism is reported. Metabolic incorporation of D 2 O into biomass in a single bacterium and the metabolic response to antibiotics are probed in as short as 10 min after culture in 70% D …

WebApr 29, 2024 · To further ensure that the Raman spectrum of each macromolecule discovered above is reproducible and accurate, we obtained in situ lipid and protein …

WebMay 20, 2024 · Deuterium Raman imaging for lipid analysis. Article. Full-text available. Oct 2024; CURR OPIN CHEM BIOL; Syusuke Egoshi; Kosuke Dodo; Mikiko Sodeoka; Raman microscopy has been used to deduce ...

WebOct 1, 2024 · Raman microscopy has been used to deduce information about the distributions of endogenous biomolecules without exogenous labeling. Several functional … hide shoe cabinetWebFeb 16, 2024 · We develop and apply super resolution bioorthogonal metabolic imaging platforms to visualize cellular metabolic dynamics in … hide sheets in excel shortcutWebDeuterium Raman imaging for lipid analysis Syusuke Egoshi1,2, Kosuke Dodo1,2 and Mikiko Sodeoka1,2 Abstract Raman microscopy has been used to deduce information … how far am i from lbiWebRaman Micro-spectral Imaging of Cells and Intracellular Drug Delivery Using Nanocarrier Systems . × Close Log In. Log in with Facebook Log in with Google. or. Email. Password. Remember me on this computer. or reset password. Enter the email address you signed up with and we'll email you a reset link. ... hide sheet tab bar in excelWebDec 17, 2015 · Deuterium-based Raman labels have been successfully used in nonlinear optical imaging ... showed an average m / z of 424 denoting an average substitution of … hide sheets with password excelWebHere we report a microscopy technique for the optical imaging, via the spectral tracing of deuterium (STRIDE), of diverse macromolecules derived from glucose. Based on stimulated Raman-scattering imaging, STRIDE visualizes the metabolic dynamics of newly synthesized macromolecules, such as DNA, protein, lipids and glycogen, via the … how far am i from king of prussiaWebDec 10, 2024 · Raman–DIP analysis at 24 h reveals that the overall active ratios in volunteer 1 and 2 were 76 ± 1.0% and 30 ± 2.0% respectively ... cm −1) at the C–D region, lipids (2874 cm −1), proteins (2928 cm −1) and DNA (2962 cm −1) at the C–H region and deuterium shifted bands at saturated lipids (1080 cm −1), carbohydrates (1097 ... how far am i from lake michigan